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DMEM, high glucose, pyruvate
Gibco DMEM高糖培养基 #11995065

产品品牌：Gibco
产品货号：11995065
产品规格：500 mL
产品价格：咨询
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产品介绍

Gibco DMEM高糖培养基#11995065

产品简介：DMEM（Dulbecco改良Eagle培养基）是一种广泛使用的基础培养基，可用于支持很多种类的哺乳动物的细胞生长。已经在DMEM中培养成功的细胞包括原代成纤维细胞、神经元、胶质细胞、HUVEC、平滑肌细胞，以及HeLa、293、Cos-7和PC-12等细胞系。

DMEM的使用：DMEM与其他培养基的不同之处在于其含有相当于初始Eagle最低必需培养基4倍浓度的氨基酸和维生素。DMEM最初采用低浓度葡萄糖(1g/L)和丙酮酸钠配制，但通常在含/不含丙酮酸钠的高糖环境下使用。DMEM不含蛋白、脂质或生长因子。因此，DMEM 需要补充剂，通常添加10%胎牛血清(FBS)。DMEM使用碳酸氢钠缓冲系统(3.7 g/L)，因此需要5–10%CO2的环境来维持生理pH值。

cGMP生产和质量体系：DMEM在位于纽约格兰德岛的符合cGMP要求的工厂内生产。该工厂是在FDA登记的医疗器械生产商，且通过ISO13485标准认证。


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