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EV-Save™ Extracellular Vesicle Blocking Reagent
FUJIFILM Wako EV-Save™ 细胞外囊泡保存稳定剂(058-09261)

产品品牌：FUJIFILM Wako
产品货号：058-09261
产品规格：1mL
产品价格：咨询
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产品介绍

FUJIFILM Wako EV-Save™ 细胞外囊泡保存稳定剂(058-09261)

产品简介：

EV-Save™细胞外囊泡保存稳定剂具有两大作用：冷冻保护和防吸附。在冷冻保护方面：由于反复冻融会使外泌体受损，因此在储存之前向含有细胞外囊泡的试管中添加EV-Save™，可以保护细胞外囊泡免受冷冻伤害，即可在-80°C下储存细胞外囊泡。在防吸附方面：在样品中添加EV-Save™，可以防止由于实验和储存过程中发生的细胞外囊泡吸附而造成的损失，提高细胞外囊泡回收率。

产品优势：

★  保护细胞外囊泡免受冷冻伤害；

★  防止细胞外囊泡在培养上清中和纯化后对实验耗材的吸附；

★  操作简单，只需往样品中添加本产品即可。

产品测试：

1. 实验条件

试用PS亲和法※1 对应试剂盒纯化得到的COLO201细胞来源外泌体进行冻融。然后使用PS Capture™ Exosome ELISA Kit （Anti Mouse IgG POD)（产品编号：297-79201）进行检测（A），以及使用透射电子显微镜（TEM）进行分析（B）。

CD63信号情况

添加EV-Save™后的情况

※1 利用磷脂酰丝氨酸（PS）结合分子以金属离子依赖性方式捕获细胞外囊泡后，用螯合剂洗脱的方法。

2. 实验结果

冻融后CD63信号减弱，但添加 EV-Save™ 后这种减弱的情况得到抑制 （图A）。此外，电子显微镜分析结果表明冻融导致了粒子数明显减少，但添加EV-Save™ 可以起到抑制粒子数减少的作用（图B）。

产品订购：

货号

产品名称

规格

058-09261

EV-Save™ Extracellular Vesicle Blocking Reagent

1mL


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HyClone Bovine Calf Serum (BCS), US Origin, 1000 mL#SH30073.04

货号：SH30073.04

HyClone Bovine Calf Serum (BCS), US Origin, 100 mL#SH30073.02

货号：SH30073.02

HyClone Bovine Calf Serum (BCS), US Origin, 500 mL#SH30073.03

货号：SH30073.03

HyClone Bovine Calf Serum (BCS), US Origin, 1000 mL, Irradiated 25-40kGy#SH30073.04IR25-40

货号：SH30073.04IR25-40

HyClone Bovine Calf Serum (BCS), US Origin, 500 mL, Irradiated 25-40kGy#SH30073.03IR25-40

货号：SH30073.03IR25-40

Gibco DMEM高糖培养基 #11995065

货号：11995065

PRECEDO膀胱癌3D类器官培养基(PRS-BLCM-3D)

货号：PRS-BLCM-3D

STEMERY人NKT细胞高效扩增试剂盒(CT-017)

货号：CT-017

STEMERY人γδT细胞高效扩增试剂盒(CT-004)

货号：CT-004

STEMERY人调节T细胞高效扩增试剂盒(CT-011)

货号：CT-011

STEMERY无血清细胞冻存液(RC-004)

货号：RC-004

Gibco澳大利亚胎牛血清(10099141C)

货号：10099141C

Gibco澳洲优质胎牛血清Fetal Bovine Serum, Premium Plus(A5669701)

货号：A5669701

STEMERY人T细胞无血清培养基(RC-003)

货号：RC-003

STEMERY人血小板裂解液(RC-002)

货号：RC-002

STEMERY人细胞毒性T细胞高效扩增试剂盒(CT-006)

货号：CT-006

STEMERY人间充质干细胞高效扩增试剂盒(CT-021)

货号：CT-021

STEMERY人树突状细胞体外快速成熟试剂盒(CT-005)

货号：CT-005

STEMERY人NK细胞高效扩增试剂盒(CT-001)

货号：CT-001

PRECEDO口腔癌3D类器官培养基(PRS-ORCM-3D)

货号：PRS-ORCM-3D

PRECEDO肺癌3D类器官培养基(PRS-LCM-3D)

货号：PRS-LCM-3D

PRECEDO宫颈癌3D类器官培养基(PRS-CCM-3D)

货号：PRS-CCM-3D

PRECEDO卵巢癌3D类器官培养基(PRS-OCM-3D)

货号：PRS-OCM-3D

PRECEDO肠癌3D类器官培养基(PRS-ICM-3D)

货号：PRS-ICM-3D

PRECEDO肝癌3D类器官培养基(PRS-HCM-3D)

货号：PRS-HCM-3D

PRECEDO乳腺癌3D类器官培养基(PRS-BCM-3D)

货号：PRS-BCM-3D

PRECEDO食管癌3D类器官培养基(PRS-ECM-3D)

货号：PRS-ECM-3D

中科普瑞昇胃癌3D类器官培养基(PRS-GCM-3D)

货号：PRS-GCM-3D