BioToolomics磁性固定化金属亲和层析填料——高质量亲和填料(190101)

产品简介：

Immobilised metal affinity chromatography (IMAC) has been widely employed as a powerful separation approach in the purification of a broad range of proteins and peptides. It is based on the specific interactions between certain transitional metal ions, mostly Cu2+, Ni2+, Zn2+ and Co2+ to the exposed amino acid surface chains containing histidine (or cysteine and tryptophane). The presence of several adjacent histidines such as (His)6-tag increases the affinity to immobilised metal ions. Increasingly, IMAC resins are employed for the purification of histidine-tagged recombinant proteins expressed in bacteria, yeast and mammalian cells. There are other applications of IMAC resins to purification of certain native non-tagged proteins as well, such as interferons, lectins, antibodies, serum and plasma proteins, peptides and peptide hormones.

Metal ions are immobilised to the carefully designed porous magnetic supports via covalently attached strong chelating groups.

产品属性：

IMAC SepFast MAG is specially designed and fabricated for magnetic purification of proteins in batch mode (i.e. stirred tank mode). The base matrix is made of cross-linked polysaccharide encapsulating with fine magnetic particles. Therefore, the resin possesses magnetic property. Removal of liquid after each step such as binding, washing and elution can be readily done by fixing the resin with a magnet.

Four types of IMAC SepFast MAG are supplied in 5 ml format with precharged metal ions as Ni SepFast MAG, Cu SepFast MAG, Zn SepFast MAG and Co SepFast MAG. A special pack that contains the above four pre-charged resins (1 ml each type) is supplied as well for the screening purpose.

Particle size	50 – 150 µm
Base matrix	Cross-linked 6% agarose encapsulating magnetic particles
Metal ion capacity	Approx. 12 – 25 µmol / ml resin*
Protein binding capacity	Depends on the type of proteins and binding conditions; could be > 40 mg / ml resin*
Chemical stability**	Stable in 0.01M HCl and 1% SDS tested for 30 mins; 0.5 M NaOH tested for overnight
pH stability**	2-14 (<2 h) 4-12 (up to one week)
Storage	20% ethanol at 4℃

产品订购：

货号	产品名称	规格
190101	Ni SepFast MAG	5 ml
190103	Co SepFast MAG	5 ml
190104	Zn SepFast MAG	5 ml