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IMAC SepFast
BioToolomics固定化金属亲和层析填料——高性价比的亲和填料(180101)

产品品牌:BioToolomics
产品货号:180101;180201;180301;180401;
产品规格:10mL;25mL;100mL;
产品价格:咨询
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产品介绍

BioToolomics固定化金属亲和层析填料——高性价比的亲和填料(180101)

产品简介:

Immobilised metal affinity chromatography (IMAC) has been widely employed as a powerful separation approach in the purification of a broad range of proteins and peptides. It is based on the specific interactions between certain transitional metal ions, mostly Ni2+, Zn2+ and Co2+ to the exposed amino acid surface chains containing histidine (or cysteine and tryptophane). The presence of several adjacent histidines such as (His)6-tag increases the affinity to immobilised metal ions. Increasingly, IMAC resins are employed for the purification of histidine-tagged recombinant proteins expressed in bacteria, yeast and mammalian cells. There are other applications of IMAC resins to purification of certain native non-tagged proteins as well, such as interferons, lectins, antibodies, serum and plasma proteins, peptides and peptide hormones. 

Metal ions are immobilised to the carefully designed porous polysaccharide polymer supports via covalently attached strong chelating groups.

产品属性:

IMAC SepFast is specifically designed and fabricated for purification of proteins in batch (stirred tank), gravity flow, or packed column mode. The base matrix is made of heavily cross-linked agarose. Its rough surface and carefully controlled pore structure allows fast access to immobilised metal ligands by target protein molecules. Also, its high mechanical strength permits liquid to pass through columns at excellent flow rates. Clarified or unclarified cell lysates (for intracellular proteins), or culture broths (for extracellular proteins), can be directly processed with IMAC SepFast. All these translate into high process flexibility and higher protein yield at shortened purification time.

Metal ions can be charged to the covalently attached chelating groups on the solid support. Leakage of metal ions is negligible.

Particle size50 – 150 µm
Base matrixCross-linked 6% agarose
Metal ion capacityApprox. 12 – 25 µmol / ml resin*
Protein binding capacity

Depends on the type of proteins and binding conditions;

could be > 40 mg / ml resin*

Chemical stability**

Stable in 0.1M HCl and 1% SDS tested for 30 mins; 

0.5 M NaOH and 30% acetic acid tested for overnight; 

0.01M HCl, 0.1M NaOH and 0.2M acetic acid tested for one week.

pH stability**2-14 (<2 h) 3-12 (up to one week)
Storage20% ethanol at 4℃

产品订购:

货号

产品名称

规格

180101-10ML


10 ml

180101-25ML

IMAC SepFast

25 ml

180101-100ML


100 ml

180201-10ML


10 ml

180201-25ML

Ni SepFast

25 ml

180201-100ML


100 ml

180301-25ML

Co SepFast

25 ml

180301-100ML


100 ml

180401-25ML

Zn SepFast

25 ml

180401-100ML


100 ml