Carboxyl activated SepFast MAG
BioToolomics羧基活化磁性琼脂糖层析填料(380201)
产品品牌:BioToolomics
产品货号:380201
产品规格:5 ml;50 ml;1 L
产品价格:咨询
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产品介绍
产品简介:
Carboxyl-activated SepFast MAG is used to make magnetic chromatography media. It forms chemically stable amide linkages with ligands containing primary amine groups. It has a long spacer (9 atoms). After binding of target molecules, the magnetic resin can be readily isolated from the biological feedstocks with the aid of a magnet. Carboxyl-activated agarose can be used for the immobilization of amine containing molecules. The coupling chemistry is carbodiimide-based and is a successful and well-documented technique.
产品属性:
Carboxyl-activated SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. It makes harsher operational conditions possible so that low pH and certain organic solvents of high concentrations can be applied. Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules. The base matrix is activated by attaching a long hydrophilic spacer arm to the backbone. It reacts directly with primary amine groups in the presence of a carbodiimide agent. Carboxyl-activated SepFast MAG is supplied as an aqueous suspension in 20% ethanol.
Group to be coupled | -NH2 |
Matrix | Highly cross-linked 4% magnetic agarose (4HF) |
Particle size* | 50 – 150 µm |
Spacer arm | 9 atoms |
Activation level | 8 - 19 µmol carboxyl / ml medium |
pH stability | 4 -13 (ligand dependent) |
Chemical stability | Compatible with all commonly used aqueous chemicals, provided the ligand to be coupled can withstand |
Storage | +4℃ - +8℃ |
产品订购:
货号 | 产品名称 | 规格 |
380201-5ML | 5 ml | |
380201-50ML | Carboxyl-activated SepFast MAG 4HF | 50 ml |
380201-1L | 1 Litre |