Carboxyl activated SepFast
BioToolomics羧基活化琼脂糖层析填料(380101)
产品品牌:BioToolomics
产品货号:380101
产品规格:5 ml;50 ml;1 L
产品价格:咨询
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产品介绍
产品简介:
Carboxyl-activated agarose beads can be used for the immobilization of amine containing molecules. The coupling chemistry is carbodiimide-based and is a successful and well-documented technique.
Carboxyl-activated SepFast forms chemically stable amide linkages with ligands containing primary amine groups. It has a long spacer (9 atoms). This pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography media for both small scale and large scale purification applications.
产品属性:
Carboxyl activated SepFast 4HF is made of highly cross-linked 4% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules.
The base matrix is activated by attaching a long hydrophilic spacer arm to the backbone. It reacts directly with the primary amine groups in molecules to be immobilized in the presence of a carbodiimide agent. Carboxyl-activated SepFast is supplied as an aqueous suspension in 20% ethanol.
Group to be coupled | -NH2 |
Matrix | Highly cross-linked 4% agarose beads (for 4HF) |
Particle size | 50 – 150 µm |
Spacer arm | 9 atoms |
Activation level | 8 - 19 µmol carboxyl / ml medium |
pH stability | 2 -13 (ligand dependent) |
Chemical stability | Compatible with all commonly used aqueous chemicals, provided the ligand to be coupled can withstand |
Storage | +4℃—+8℃ |
产品订购:
货号 | 产品名称 | 规格 |
380101-5ML | 5 ml | |
380101-50ML | Carboxyl-activated SepFast 4HF | 50 ml |
380101-1L | 1 Litre |