苯基疏水层析填料

Product Introduction:

Butyl, Phenyl and Octyl SepFast are hydrophobic interaction chromatography (HIC) adsorbents having a carbon chain of C4, benzene ring and C8, respectively. They are specially designed for the purification of biological molecules based on their hydrophobicity profiles.

HIC is a versatile technique and could show high selectivity to individual molecules according to their exposed hydrophobic zones. It is particularly useful for intermediate and final-stage purifications. A HIC medium normally binds at moderate to high salt concentrations. It is logical to place HIC step after an IEX step where molecules are usually eluted at high salt conditions.

HIC media shows much milder purification conditions than reversed phase chromatography (RPC) media. Better biological activity could be maintained in HIC operations than RPC operations. SepFast HIC media offers a broad range of choices. Generally speaking, the longer the carbon chain, the higher the surface hydrophobicity. The base matrix is made of agarose that has been highly cross-linked. It is very stable to most of the chemical conditions experienced in bioprocessing industry. Process developers and manufacturers have the chance to choose the best HIC media for their applications. The core advantages are:

• High sample loading capacity

• More choices than other suppliers

• High separation power

• All versions can be scaled up and produced for GMP use

• Regulatory support files available

Product Properties:

Matrix	4% agarose for SepFast 4HF; 6% agarose for SepFast 6HF
Functional group	-C6H6
Ligand density	Around 25 μmol/ml Around 40 μmol/ml (high sub)
Binding capacity	>20 mg BSA / ml
Particle size	25 - 50 μm (HighRes), 50 - 150 μm (4HF and 6HF), 150 - 350 μm (Large Beads)
Pressure-flow property*	>100 cm/h for HighRes; >300 cm/h for 4HF serials; >500 cm/h for 6HF serials; >1500 cm/h for Large Beads
Operational pressure	Up to 3 bar
pH stability	2-14 (short term) and 3-12 (long term)
Working temperature	+4℃ to +30℃
Chemical stability	All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% acetonitrile, 30% isopropanol, 70% ethanol, 3 M (NH4)2SO4
Storage	20% ethanol

Product Series:

Description	Quantity	Code no.
Phenyl SepFast 4HF	25 ml	481001-25ML
	100 ml	481001-100ML
	250 ml	481001-250ML
	500 ml	481001-500ML
	1 Litre	481001-1L
	5 Litre	481001-5L
	10 Litre	481001-10L
Phenyl SepFast 6HF	25 ml	481201-25ML
	100 ml	481201-100ML
	250 ml	481201-250ML
	500 ml	481201-500ML
	1 Litre	481201-1L
	5 Litre	481201-5L
	10 Litre	481201-10L
Phenyl SepFast 6HF High Sub	25 ml	481202-25ML
	100ml	481202-100ML
	250 ml	481202-250ML
	500 ml	481202-500ML
	1 Litre	481202-1L
	5 Litre	481202-5L
	10 Litre	481202-10L

Phenyl SepFast Large Bead	25 ml	481301-25ML
	100 ml	481301-100ML
	250 ml	481301-250ML
	500 ml	481301-500ML
	1 Litre	481301-1L
	5 Litre	481301-5L
	10 Litre	481301-10L
Phenyl SepFast HighRes	25 ml	481101-25ML
	100 ml	481101-100ML
	250 ml	481101-250ML
	500 ml	481101-500ML
	1 litre	481101-1L
	5 Litre	481101-5ML
	10 Litre	481101-25ML