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Butyl SepFast Media
BioToolomics丁基疏水层析填料(480101)

产品品牌:BioToolomics
产品货号:480101;480201;480301;
产品规格:25mL;100mL;250mL;500mL;1L;5L;10L
产品价格:咨询
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产品介绍

BioToolomics丁基疏水层析填料(480101)

产品简介:

Butyl, Phenyl and Octyl SepFast are hydrophobic interaction chromatography (HIC) adsorbents having a carbon chain of C4, benzene ring and C8, respectively. They are specially designed for the purification of biological molecules based on their hydrophobicity profiles. 

HIC is a versatile technique and could show high selectivity to individual molecules according to their exposed hydrophobic zones. It is particularly useful for intermediate and final-stage purifications. A HIC medium normally binds at moderate to high salt concentrations. It is logical to place HIC step after an IEX step where molecules are usually eluted at high salt conditions. 

HIC media shows much milder purification conditions than reversed phase chromatography (RPC) media. Better biological activity could be maintained in HIC operations than RPC operations. SepFast HIC media offers a broad range of choices. Generally speaking, the longer the carbon chain, the higher the surface hydrophobicity. The base matrix is made of agarose that has been highly cross-linked. It is very stable to most of the chemical conditions experienced in bioprocessing industry. Process developers and manufacturers have the chance to choose the best HIC media for their applications. The core advantages are:

       •  High sample loading capacity 

       •  More choices than other suppliers 

       •  High separation power 

       •  All versions can be scaled up and produced for GMP use 

       •  Regulatory support files available

产品属性:


Matrix

Highly cross-linked agarose;

4% agarose for SepFast 4HF;

6% agarose for SepFast 6HF

Functional group-(CH2)3CH3
Ligand density

Around 40 μmol/ml

Binding capacity>20 mg lyzosyme / ml
Particle size

25 - 50 μm (HighRes), 50 - 150 μm (4HF and 6HF),

150 - 350 μm (Large Beads)


Pressure-flow property*

>100 cm/h for HighRes; 

>300 cm/h for 4HF serials; 

>500 cm/h for 6HF serials; 

>1500 cm/h for Large Beads

Operational pressureUp to 3 bar
pH stability2-14 (short term) and 3-12 (long term)
Working temperature+4℃ to +30℃


Chemical stability

All commonly used buffers;

1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 

30% acetonitrile, 30% isopropanol, 70% ethanol, 3 M (NH4)2SO4

Storage20% ethanol

产品订购:

货号

产品名称

规格

480101-25ML


25 ml

480101-100ML


100 ml

480101-1L

Butyl SepFast 4HF

1 Litre

480101-5L


5 Litre

480101-10L


10 Litre

480301-25ML


25 ml

480301-100ML


100 ml

480301-1L

Butyl SepFast 6HF

1 Litre

480301-5L


5 Litre

480301-10L


10 Litre

480201-25ML


25 ml

480201-100ML

Butyl SepFast Large Beads

100 ml

480201-1L


1 Litre