Amine activated SepFast MAG
Amine-activated SepFast MAG is used to make magnetic chromatography adsorbents. It forms chemically stable amide linkages with ligands containing carboxyl groups. It has a long spacer (9 atoms). After binding of target molecules, the magnetic resin can be readily isolated from the biological feedstocks with the aid of a magnet.
Amine-activated agarose can be used for the immobilization of carboxyl containing, or aldehyde containing molecules. The coupling chemistry is carbodiimide-based or reduced amination based and is a successful and well-documented technique.
Amine-activated SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. It makes harsher operational conditions possible, so that low pH and certain organic solvents of high concentrations can be applied.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules.
The base matrix is activated by attaching a long hydrophilic chain with an amine group in the end. They react with either carboxyl containing ligands in the presence of a carbodiimide agent, or aldehyde containing ligands in the presence of a reducing agent. Amine-activated SepFast is supplied as an aqueous suspension in 20% ethanol.
Group to be coupled
Highly cross-linked 4% magnetic agarose (4HF)
50 – 150 µm
10 - 25 µmol amine / ml medium
4 -13 (ligand dependent)
Compatible with all commonly used aqueous chemicals, provided the ligand to be coupled can withstand.
Amine-activated SepFast MAG 4HF