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NHS-activated SepFast
BioToolomics NHS活化琼脂糖层析填料(330101)

产品品牌:BioToolomics
产品货号:330101
产品规格:5 ml;50 ml;1 L
产品价格:咨询
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产品介绍

NHS活化琼脂糖层析填料

产品简介:

NHS (N-hydroxysuccinimide)-activated agarose has a well-proven track record for the preparation and use of custom affinity chromatography media. Coupling biospecific ligands to NHS-activated agarose is a successful and well-documented technique. The coupling reaction is spontaneous, rapid and easy to carry out. No toxic chemicals or special equipment is required.

NHS-activated SepFast media forms chemically stable amide bonds with ligands containing primary amine groups. The activated NHS group has a long spacer arm (11 atoms) that is particularly helpful in the immobilization of structurally restricted ligands such as proteins and peptides. This pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography adsorbents for both small scale and large scale purification applications.

NHS活化琼脂糖层析填料图片

产品属性:

NHS activated SepFast 4HF is made of highly cross-linked 4% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure in a packed column.

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.

The base matrix is activated through a long hydrophilic spacer arm (11 atoms) with N-hydroxysuccinimide at the end. It reacts directly with the primary amine groups in molecules to be immobilized to form stable amide bonds. NHS-activated SepFast media is supplied as a suspension in 100% isopropanol.

Group to be coupled

-NH2

Matrix

SepFast 4HF: highly cross-linked 4% agarose

Particle size

50 – 150 µm

Activation level

5 - 19 µmol NHS / ml medium

pH stability

3 -13 (ligand dependent)

Chemical stability

Compatible with all commonly used aqueous chemicals, provided the ligand to be coupled can withstand.

Storage

+2℃—+8℃

产品订购:

货号

产品名称

规格

330101-5ML


5 ml

330101-50ML

NHS-activated SepFast 4HF

50 ml

330101-1L


1 Litre


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