BioToolomics谷胱甘肽亲和层析填料——专业纯化介质(320101)

产品简介：

Glutathione SepFast is an affinity chromatography medium used for the rapid one-step purification of Glutathione S-transferase (GST)-tagged proteins. Other Glutathione S-transferases and Glutathione-binding proteins can also be purified with this adsorbent. 

Reduced Glutathione ligand is immobilised to the carefully designed porous support via very stable thiolether linkages. GST-tagged proteins expressed in bacteria, yeasts, insects and mammalian cell cultures can be readily purified in a single step. The GST tag can be cleaved in a bound condition or in an eluted condition by specific proteases.

产品属性：

Glutathione SepFast adsorbents are specifically designed and fabricated for the purification of GST-tagged proteins in batch (stirred tank), gravity flow or packed column modes. Glutathione SepFast is made of highly cross-linked agarose beads. Its carefully controlled pore structure allows fast access to affinity ligands by target protein molecules. Also, its high mechanical strength permits liquid to pass through gravity columns or packed columns at excellent flow rates. Clarified or unclarified cell lysates (for intracellular proteins), or culture broths (for extracellular proteins) could be directly processed with Glutathione SepFast. All these translate into high process flexibility and higher protein yield at shortened purification time.

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