0.67ml层析空柱

产品介绍：目前市场上的预填充柱具有较差的可扩展性和较差的机械强度。由于壁支撑效应和单个色谱树脂的刚性，在流动填料下沉后，颗粒也能正确压缩，然后会形成一个与放大柱相关的可预测色谱性能的紧密填充床。

BioToolomics提供的一次性色谱柱系列具有可重复使用的柱，但也具有许多其他优点，例如；树脂比重压缩性、一次性、易于包装、任何类型色谱介质的紧密包装（>20µm），与放大柱相同的设计，坚固、精确和可重复的填料，允许适用于中等背压，易于储存，使用寿命长。

在选定的填料填充在柱中之后，柱可以储存并多次使用。

柱部件主要由聚丙烯制成，这种材料对大多数常用试剂表现出优异的耐化学性（见下面的化学兼容性表）。柱具有标准连接，即与常用色谱仪器（如AKTA）兼容。

推荐的操作压力可高达5 bar（大多数工艺色谱介质允许工作压力小于3bar）。

柱部件说明：

化学兼容性表：

Packing the column：

•  Connect each End Plug to each Extension Plug.  

•  Insert one of the above assemblies into one end of the Column Body. Push the End Plug until it is fully engaged with the shoulder of the Column Body. 

•  Screw a 10-32 male/luer female connector to the End Plug. Use a syringe pre-filled with 1 – 2 ml of water to fill the column to a level of 0.2 – 0.3 ml. 

•  Pipette the resin slurry in and suck the bed down with the syringe. Be careful not to dry the bed. If necessary, pipette in more water or more slurry or pipette out more slurry until the packed volume (under suction) reaches the desired level. Note: the packed volume depends on the type of resin. As a guide, the packed volume should be 0.4 – 0.5 ml for agarose-based spherical particles. 

•  Pipette water in to fully fill the column. Carefully insert the other assembly (to avoid trapping of air bubble). Push it down slowly until the liquid level reaches the thread. Stop pushing and screw a stop plug to seal the top. Then push the top End Plug down until it is fully engaged in the locking mechanism. Note: don’t remove the syringe in this stage. 

•  Remove the 10-32 male/luer female connector and the syringe. Screw another Stop Plug into the bottom End Plug. 

•  Depending on the nature of individual resins, this step may be operated to further settle the particles in the bed. Pre-fill a syringe with 10 ml liquid (ideally the same liquid as the one in the column). Insert it into a 10-32 male/luer female connector and purge out any air in the flow path. Remove the top Stop Plug. Attach the pre-filled syringe to the top End Plug (be sure that no air is trapped in the flow path). Remove the bottom Stop Plug. Push through at least 3 ml of liquid under pressure (e.g as fast as possible) by hand. Seal the bottom with a Stop Plug. Disconnect the syringe and then seal the top. This step can also be done by connecting the column to a chromatography system (such as AKTA) to pump liquid through at high speed (e.g. 3-5 ml/min).