Protein A SepFast Range
BioToolomics Protein A亲和层析填料——抗体纯化亲和填料(230801)
产品品牌:BioToolomics
产品货号:230801;230901;230101;230301;230201;230401
产品规格:5mL;25mL;100mL;250mL;500mL;1L;5L;10L
产品价格:咨询
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产品介绍
产品简介:
Protein A SepFast range is affinity chromatography media for the purification of immunoglobulins. Its purification power has been well documented in various antibody purification applications, such as isolation and purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media.
The range of Protein A SepFast media is developed and supported for production scale chromatography use. Regulatory Support File (RSF) is available to assist process validation and submissions to regulatory authorities.
Protein A SepFast: Suitable for most application from small scale to large bioprocessing scale
Protein A SepFast HighRes: Suitable for processing small quantity of antibodies at reduced time
Protein A SepFast Large Bead: Suitable for processing crude or very viscous feedstocks
Protein A SepFast Endure range is affinity chromatography media for the purification of immunoglobulins, such as isolation and purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The ligand is specially designed allowing to tolerate higher alkaline concentrations during the cleaning and regeneration step. The protein A Endure ligand binds to the Fc region of immunoglobulins. The binding is highly specific so high purity can be achieved in a single step.
Protein A SepFast Endure: Suitable for most application from small scale to large bioprocessing scale
Protein A SepFast HighRes Endure: Suitable for processing small quantity of antibodies at reduced time
Protein A SepFast Large Bead Endure: Suitable for processing crude or very viscous feedstocks
The range of Protein A SepFast Endure is developed and supported for production scale chromatography use. Regulatory Support File (RSF) is available to assist process validation and submissions to regulatory authorities.
产品属性:
Protein A is immobilised to highly porous and highly cross-linked agarose base matrix. Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low nonspecific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules. The medium shows high mechanical rigidity, so it can be operated at moderate to high flow velocities with moderate pressure drops.
Matrix | Highly cross-linked Agarose |
Particle size | 50 - 150 µm (Protein A SepFast) 20 - 50 µm (Protein A SepFast HighRes) 150 - 350 µm (Protein A SepFast Large Bead) 50 - 150 µm (Protein A SepFast Endure) 20 - 50 µm (Protein A SepFast HighRes Endure) 150 - 350 µm (Protein A SepFast Large Bead Endure) |
Binding capacity | >30 mg/ml human IgG/ml |
Operational pressure | Up to 3 bar (42 psi) |
pH stability | 2-10 (short term) and 3-9 (long term) |
Working temperature | +4℃ to +30℃ |
Chemical stability | Compatible with most commonly used reagents for antibody purifications |
Sanitisation | Wash the packed column with 2% hibitane/20% ethanol |
Storage | 20% ethanol at +4℃- +8℃ |
产品订购:
货号 | 产品名称 | 规格 |
230801-5ML | 5 ml | |
230801-25ML | 25 ml | |
230801-100ML | Protein A SepFast HighRes | 100 ml |
230801-250ML | 250 ml | |
230801-500ML | 500 ml | |
230801-1L | 1 litre | |
230901-5ML | 5 ml | |
230901-25ML | 25 ml | |
230901-100ML | Protein A SepFast HighRes Endure | 100 ml |
230901-250ML | 250 ml | |
230901-500ML | 500 ml | |
230901-1L | 1 litre | |
230101-5ML | 5 ml | |
230101-25ML | 25 ml | |
230101-100ML | 100 ml | |
230101-250ML | Protein A SepFast | 250 ml |
230101-500ML | 500 ml | |
230101-1L | 1 litre | |
230101-5L | 5 L | |
230101-10L | 10 L | |
230301-5ML | 5 ml | |
230301-25ML | 25 ml | |
230301-100ML | 100 ml | |
230301-250ML | Protein A SepFast Endure | 250 ml |
230301-500ML | 500 ml | |
230301-1L | 1 litre | |
230301-5L | 5 L | |
230301-10L | 10 L | |
230201-5ML | 5 ml | |
230201-25ML | 25 ml | |
230201-100ML | 100 ml | |
230201-250ML | Protein A SepFast Large Bead | 250 ml |
230201-500ML | 500 ml | |
230201-1L | 1 litre | |
230201-5L | 5 L | |
230201-10L | 10 L | |
230801-5ML | 5 ml | |
230401-25ML | 25 ml | |
230401-100ML | 100 ml | |
230401-250ML | Protein A SepFast Large Bead Endure | 250 ml |
230401-500ML | 500 ml | |
230401-1L | 1 litre | |
230401-5L | 5 L | |
230401-10L | 10 L |