BioToolomics蛋白G亲和层析填料————-高性能亲和层析填料(230501)

产品简介:

Protein G SepFast range is affinity resins with protein G immobilized to highly cross-linked agarose-based SepFast beads.

Protein G binds to the Fc region of IgG from a variety of mammalian species. Protein G SepFast may be used to isolate and purify classes, subclasses and fragments of immunoglobins from any biological fluid or cell culture medium. Protein G SepFast is extremely useful for isolation of immune complexes.

The potential applications of protein G include practically all of the current and projected applications of protein A. Protein G and protein A, however, have different IgG binding specificities, dependent on the origin of the IgG. Compared to protein A, protein G binds more strongly to polyclonal IgG, for example, from cow, sheep and horse. Furthermore, unlike protein A, protein G binds polyclonal rat IgG, human IgG3 and mouse IgG1.

The range of Protein G SepFast media is developed and supported for production scale chromatography. Regulatory Support File (RSF) ≥is available to assist process validation and submissions to regulatory authorities.

Protein G SepFast: Suitable for most application from small scale to large bioprocessing scale

Protein G SepFastHighRes: Suitable for processing small quantity of antibodies at reduced time

Protein G SepFast Large Bead: Suitable for processing crude or very viscous feedstocks

产品属性:

Matrix	Highly cross-linked agarose bead
Product format	White to creamy slurry
Binding capacity*	≥ 15 mg human IgG/mL resin
Particle size	50 - 150 μm (Protein G SepFast ) 20 - 50 μm (Protein G SepFast HighRes) 150 - 350 μm (Protein G SepFast Large Bead)
Recommended flow rate**	50 to 300 cm/h
Max. operating pressure	0.2 MPa (2 bar, 28 psi)
Chemical stability	Stable in most commonly used buffers, 6 M guanidine-HCl; 70% ethanol. 8 M urea, pH 10.5; 0.1 M Glycine-NaOH, pH 11.
Physical stability	Negligible volume variation due to changes in pH or ionic strength.
pH stability	3 to 9 (long term); 2 to 10 (short term)
Sanitization	Sanitize the column with 70% ethanol
Storage temperature	20% ethanol at 2°C to 8°C

产品订购:

货号	产品名称	规格
230701-5ML		5 ml
230701-25ML		25 ml
230701-100ML	Protein G SepFast HighRes	100 ml
230701-250ML		250 ml
230701-500ML		500 ml
230701-1L		1 litre
230501-5ML		5 ml
230501-25ML		25 ml
230501-100ML	Protein G SepFast	100 ml
230501-250ML		250 ml
230501-500ML		500 ml
230501-1L		1 litre
230601-5ML		5 ml
230601-25ML		25 ml
230601-100ML	Protein G SepFast Large Bead	100 ml
230601-250ML		250 ml
230601-500ML		500 ml
230601-1L		1 litre